Submission Template
ResearchHub Journal (RHJ)
Please use this template to prepare your manuscript for submission to ResearchHub. Remove any italicized instructions before submission.
Title of the Manuscript
Provide a concise and informative title that reflects the content of your manuscript.
Authors and Affiliations
List all authors (Name Middle initial. Last name) and their affiliations.
First Author¹, Second Author², Third Author³*
¹Department, Institution, City, Country
²Department, Institution, City, Country
³Department, Institution, City, Country
*Corresponding author: [email protected]Abstract
Provide a structured abstract of up to 250 words summarizing the Background, Methods, Results, and Conclusions of the study. Refer to the following resource for best practices: https://www.nature.com/documents/nature-summary-paragraph.pdf
Introduction
Provide a background and clearly state the research question, hypothesis or objectives, and significance of the study with reference to previous work.
Materials and Methods
Provide detailed information to allow replication of the study.
Study Design
Describe the type of study and its design.
Participants or Subjects
Human Subjects — Specify:
Selection and exclusion criteria
Sample size calculation and justification
Demographic characteristics
Recruitment methods
Ethical approvals and institutional review board numbers
Informed consent procedures
Group allocation methods
Animal Models — Detail:
Species, strain, and genetic background
Age, sex, and weight ranges
Source/vendor and stock numbers
Housing and husbandry conditions
Breeding schemes and genetic crosses
Genotyping procedures
Animal health status and monitoring
Euthanasia methods
Number of animals per experimental group
Cell Lines — Specify:
Cell line name and source/repository
Authentication methods
Mycoplasma testing status
Passage numbers used
Culture conditions (media, supplements, environment)
Cell harvesting and maintenance protocols
Quality control measures
Other Model Systems — Include relevant details for:
Bacterial/yeast strains
Plant varieties
Organoid systems
Tissue explants
Computational models
For clinical trials, include the registration number and registry name.
Materials
All materials used in the study must be described with sufficient detail to enable reproducibility. Include:
Chemical compounds: Full chemical names, purity grades, CAS numbers (where applicable), and manufacturer details
Biological materials: Species, strain designations, source/supplier, authentication methods, and relevant identifiers
Engineering materials/equipment: Model numbers, specifications, manufacturer information, and critical operating parameters
Software/computational tools: Version numbers, settings, and relevant parameters
For novel materials or methods, provide detailed preparation protocols. For well-established materials and methods, cite appropriate references. Include any safety considerations or special handling requirements when relevant. All materials must comply with relevant safety and ethical guidelines.
Data Collection
Explain the methods and tools used for data collection by specifying:
Measurement techniques and instruments
Calibration procedures
Sampling intervals and conditions
Quality control measures
Data recording methods and formats
Validation procedures
Observer training (if applicable)
Interventions or Procedures
Describe any interventions or experimental procedures by specifying:
Step-by-step experimental protocols
Control and treatment conditions
Duration and frequency of interventions
Equipment settings and parameters
Environmental conditions
Quality assurance measures
Modifications to standard protocols (if any)
Data Analysis
Describe the statistical methods used and the allowances made (if any) for controlling bias or unwanted sources of variability. Include:
Statistical tests employed with justification
Software packages and versions used
Data preprocessing steps
Handling of missing data
Methods for controlling bias
Power analysis (if applicable)
Effect size calculations
Confidence intervals
Statistical significance criteria
Methods for handling outliers
Validation procedures for analytical methods
Results and Discussion
Present the findings logically, using text, tables, and figures as appropriate.
Main Findings
Detail the primary outcomes of the study.
Discussion
Interpret the results in the context of existing literature.
Key Insights
Highlight the significance and implications of the findings.
Limitations
Discuss any limitations of the study.
Conclusions
Summarize the main findings and their potential impact.
Acknowledgments
Recognize individuals or organizations that assisted in the study but are not listed as authors.
Example:
"The authors thank Jane Doe for her assistance with data collection."
Funding
Disclose all sources of funding for the research.
Example:
"This work was supported by the National Science Foundation [Grant No. XXXX]."
If not applicable, add: "This work received no specific grant funding."
Author Contributions
Specify the contributions of each author using their initials.
Example:
A.B. conceived and designed the experiments.
C.D. performed the experiments.
E.F. analyzed the data.
A.B. and E.F. wrote the manuscript.
All authors read and approved the final manuscript.
Data and Code Availability
State where the data supporting the findings can be found. We highly recommend leveraging existing repositories such as Zenodo for open access to data and GitHub for open source software access alongside your publication.
Data: CC0 or CC-BY
Code: MIT, Apache, or similar open source license
Examples:
"The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request."
"All data are included in the manuscript and supplementary materials."
"Data is available in a public repository [provide DOI or URL]."
Conflicts of Interest
This section is mandatory. All authors must disclose any financial or personal relationships that could influence the work.
Example (no conflicts):
"The authors declare that they have no conflicts of interest."
Example (with conflicts):
"Author A.B. has received research grants from Company X. Author C.D. is a consultant for Company Y."
Ethical Approval
Include the name of the ethics committee that approved the study and the approval number.
Human Subject Research:
"This study was approved by the Institutional Review Board of [Institution Name] (approval number: XXX-YYYY). All participants provided written informed consent before enrollment."
Animal Research:
"All animal procedures were approved by the Institutional Animal Care and Use Committee of [Institution Name] (protocol number: IACUC-XXXX) and conducted in compliance with the Guide for the Care and Use of Laboratory Animals."
Consent to Participate
State that informed consent was obtained from all participants.
Example:
"Informed consent was obtained from all individual participants included in the study."
If not applicable, add: "Ethics and consent were not required for the performed study."
References
List all references cited in the text in a consistent citation style (Vancouver). Ensure all references are complete and accurate.
Example format:
Author(s). Title of the article. Journal Name. Year; Volume(Issue). DOI
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