# Submission Template Please use this template to prepare your manuscript for submission to ResearchHub. Remove any italicized instructions before submission. ### Title of the Manuscript Provide a concise and informative title that reflects the content of your manuscript. *** ### Authors and Affiliations List all authors (Name Middle initial. Last name) and their affiliations. ``` First Author¹, Second Author², Third Author³* ¹Department, Institution, City, Country ²Department, Institution, City, Country ³Department, Institution, City, Country *Corresponding author: email@example.com ``` *** ### Abstract Provide a structured abstract of up to 250 words summarizing the Background, Methods, Results, and Conclusions of the study. Refer to the following resource for best practices: *** ### Introduction Provide a background and clearly state the research question, hypothesis or objectives, and significance of the study with reference to previous work. *** ### Materials and Methods Provide detailed information to allow replication of the study. #### Study Design Describe the type of study and its design. #### Participants or Subjects **Human Subjects** — Specify: * Selection and exclusion criteria * Sample size calculation and justification * Demographic characteristics * Recruitment methods * Ethical approvals and institutional review board numbers * Informed consent procedures * Group allocation methods **Animal Models** — Detail: * Species, strain, and genetic background * Age, sex, and weight ranges * Source/vendor and stock numbers * Housing and husbandry conditions * Breeding schemes and genetic crosses * Genotyping procedures * Animal health status and monitoring * Euthanasia methods * Number of animals per experimental group **Cell Lines** — Specify: * Cell line name and source/repository * Authentication methods * Mycoplasma testing status * Passage numbers used * Culture conditions (media, supplements, environment) * Cell harvesting and maintenance protocols * Quality control measures **Other Model Systems** — Include relevant details for: * Bacterial/yeast strains * Plant varieties * Organoid systems * Tissue explants * Computational models For clinical trials, include the registration number and registry name. #### Materials All materials used in the study must be described with sufficient detail to enable reproducibility. Include: * **Chemical compounds:** Full chemical names, purity grades, CAS numbers (where applicable), and manufacturer details * **Biological materials:** Species, strain designations, source/supplier, authentication methods, and relevant identifiers * **Engineering materials/equipment:** Model numbers, specifications, manufacturer information, and critical operating parameters * **Software/computational tools:** Version numbers, settings, and relevant parameters For novel materials or methods, provide detailed preparation protocols. For well-established materials and methods, cite appropriate references. Include any safety considerations or special handling requirements when relevant. All materials must comply with relevant safety and ethical guidelines. #### Data Collection Explain the methods and tools used for data collection by specifying: * Measurement techniques and instruments * Calibration procedures * Sampling intervals and conditions * Quality control measures * Data recording methods and formats * Validation procedures * Observer training (if applicable) #### Interventions or Procedures Describe any interventions or experimental procedures by specifying: * Step-by-step experimental protocols * Control and treatment conditions * Duration and frequency of interventions * Equipment settings and parameters * Environmental conditions * Quality assurance measures * Modifications to standard protocols (if any) #### Data Analysis Describe the statistical methods used and the allowances made (if any) for controlling bias or unwanted sources of variability. Include: * Statistical tests employed with justification * Software packages and versions used * Data preprocessing steps * Handling of missing data * Methods for controlling bias * Power analysis (if applicable) * Effect size calculations * Confidence intervals * Statistical significance criteria * Methods for handling outliers * Validation procedures for analytical methods *** ### Results and Discussion Present the findings logically, using text, tables, and figures as appropriate. #### Main Findings Detail the primary outcomes of the study. #### Discussion Interpret the results in the context of existing literature. #### Key Insights Highlight the significance and implications of the findings. #### Limitations Discuss any limitations of the study. *** ### Conclusions Summarize the main findings and their potential impact. *** ### Acknowledgments Recognize individuals or organizations that assisted in the study but are not listed as authors. **Example:** > "The authors thank Jane Doe for her assistance with data collection." *** ### Funding Disclose all sources of funding for the research. **Example:** > "This work was supported by the National Science Foundation \[Grant No. XXXX]." If not applicable, add: "This work received no specific grant funding." *** ### Author Contributions Specify the contributions of each author using their initials. **Example:** * A.B. conceived and designed the experiments. * C.D. performed the experiments. * E.F. analyzed the data. * A.B. and E.F. wrote the manuscript. * All authors read and approved the final manuscript. *** ### Data and Code Availability State where the data supporting the findings can be found. We highly recommend leveraging existing repositories such as [Zenodo](https://zenodo.org/) for open access to data and [GitHub](https://github.com/) for open source software access alongside your publication. * **Data:** CC0 or CC-BY * **Code:** MIT, Apache, or similar open source license **Examples:** > "The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request." > "All data are included in the manuscript and supplementary materials." > "Data is available in a public repository \[provide DOI or URL]." *** ### Conflicts of Interest This section is mandatory. All authors must disclose any financial or personal relationships that could influence the work. **Example (no conflicts):** > "The authors declare that they have no conflicts of interest." **Example (with conflicts):** > "Author A.B. has received research grants from Company X. Author C.D. is a consultant for Company Y." *** ### Ethical Approval Include the name of the ethics committee that approved the study and the approval number. **Human Subject Research:** > "This study was approved by the Institutional Review Board of \[Institution Name] (approval number: XXX-YYYY). All participants provided written informed consent before enrollment." **Animal Research:** > "All animal procedures were approved by the Institutional Animal Care and Use Committee of \[Institution Name] (protocol number: IACUC-XXXX) and conducted in compliance with the Guide for the Care and Use of Laboratory Animals." *** ### Consent to Participate State that informed consent was obtained from all participants. **Example:** > "Informed consent was obtained from all individual participants included in the study." If not applicable, add: "Ethics and consent were not required for the performed study." *** ### References List all references cited in the text in a consistent citation style (Vancouver). Ensure all references are complete and accurate. **Example format:** > 1. Author(s). Title of the article. Journal Name. Year; Volume(Issue). DOI